Plasmids for Exosome Research

Exosomes are a class of membrane-bound extracellular vesicles (EVs) with a size range of ~30 to 160 nm (average ~100 nm) in diameter. Most eukaryotic cells, including in vitro cultured cells, can release exosomes.

The biogenesis of exosomes starts with double invagination of the plasma membrane and the formation of intracellular multivesicular bodies (MVBs) containing intraluminal vesicles (ILVs). The ILVs become exosomes when the MVB fuses with the plasma membrane and secret the vesicles into the extracellular space through exocytosis.

Exosomes contain many molecular constituents of their cell of origin, including lipids, DNA, RNA, metabolites, and cytosolic and cell-surface proteins. The membranes of exosomes contain some transmembrane proteins of the original cell membrane, which often includes CD9, CD63, and CD81.

There is a growing interest in studying the roles of exosomes and develop exosome-based diagnostic and therapeutic tools for the treatment of many diseases. RGBiotech offers plasmids that express exosome markers for supporting your exosome research. These plasmids fuse the exosome marker with a fluorescent protein under the control of multiple promoters. Cells transfected with these plasmids are able to secret fluorescent labelled exosomes, allowing researchers to easily monitor exosome dynamics and functional studies in real time. If your target cells are hard to transfect, we also provide lentiviral or retroviral based plasmids. We can also offer custom design and construction services. Please contact us at admin@rgbiotech.com for more information.

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