Glutamate Sensor Plasmids

Glutamate is a neurotransmitter that plays a variety of roles in synaptic communication. Scientists have developed glutamate-sensitive fluorescent reporters (GluSnFR) to monitor glutamate release from neurons in vivo by fluorescence microscopy.

The widely used SF-iGluSnFR consists of a circularly permuted consists of a circularly permuted superfolder GFP fused to a glutamate binding protein (GluBP) from a bacterium. GluBP can change its shape when it binds to glutamate. The conformational change could pull the GFP barrel together which increases fluorescence.

RGBiotech offers a list of SF-iGluSnFR plasmids with different promoters, 1) constitutive promoter CMV and CAG, 2) neuron-specific expression promoter human Synapsin-1, 3) astrocyte-specific expression promoter GFAP.

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