Cre-Dependent GluN2B Knockdown Tool for Precision Neural Research

RGBiotech offers pAAV-CMV-dsRed-pSico-GFP-GluN2BshRNA plasmid vector - a state-of-the-art tool designed for Cre-dependent knockdown of mouse GluN2B. This plasmid vector utilizes AAV expression vector backbone that allows further production of high titers of Adeno-associated viruses (AAV) particles with serotype of interest. Please contact us at admin@rgbiotech.com for pricing and more information.

How It Works?

The AAV1-CMV-dsRed-pSico-GluN2BshRNA plasmid incorporates a sophisticated dual-fluorescence reporter system (dsRed and GFP) that provides real-time, visual validation of two critical experimental milestones: successful transduction and Cre-mediated induction of mouse GluN2B shRNA expression.
The dsRed fluorescent protein is expressed constitutively under the control of the CMV promoter, independent of Cre recombination. Its persistent expression allows for straightforward quantification of transfection efficiency in vitro or transduction efficiency in vivo following AAV delivery. The bright, stable dsRed signal enables researchers to accurately identify and confirm which cells or tissue regions have received the vector, ensuring robust and interpretable experimental results.
The GFP reporter gene is positioned within the loxP-flanked "STOP" cassette that silences the U6-shRNA expression module. In its default state (pre-recombination), cells expressing dsRed will also express GFP, resulting in a dual-fluorescent population. Upon delivery of Cre recombinase, the excision of this STOP cassette simultaneously activates the transcription of the mouse GluN2B-specific shRNA and permanently disrupts the GFP reporter gene. Consequently, the loss of GFP fluorescence serves as a direct and intuitive visual indicator of successful Cre-mediated recombination and, by extension, the active expression of the GluN2B-targeting shRNA.

Advantages

Applications

Frequently Asked Questions (FAQs)

Q1: How do I confirm GluN2B knockdown efficiency?
A1: We recommend combining fluorescence imaging (GFP/dsRed) with molecular validation: qRT-PCR for GluN2B mRNA levels and Western blot for protein expression.

Q2: Can this vector be used in non-neuronal cells?
A2: Yes-while GluN2B is most abundant in neurons, it is also expressed in astrocytes and microglia.

Q3: Is the vector suitable for in vivo studies?
A3: Absolutely. This plasmid vector can be packaged into AAV particles which are ideal for in vivo use due to their low immunogenicity and long-term transgene expression.

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