Inducible Expression Solutions

RGBiotech offers products and services for biological research. Since the day of establishment, we has been striving to provide best products and services with competitive prices and fast turnaround time to customers all over the world. Our commitment is to do our best on each product and each project. We are happy and dedicated to become an indispensible partner on the way of discovering new biological mechanisms and improving human/animal health.

1.Tetracycline Inducible Expression System

The tetracycline inducible expression system is a powerful tool that can help researchers reversibly turn on or turn off gene transcription in the presence of the antibiotic tetracycline or its derivatives doxycycline (DOX). This system consists of a cis Tet Response Element (TRE) placed upstream of a minimal promoter and a trans activator or repressor that can bind to TRE.

1)Tet Response Element (TRE)

The TRE is seven repeats of a 19 bp bacterial tetracycline operator (tetO) sequence [TCCCTATCAGTGATAGAGA] separated by spacer sequences.

2)Tetracycline Off System

Tetracycline Off (Tet-Off) System is first developed by Gossen and Bujard. Tet-Off System involves a tetracycline-controlled transactivator (tTA) which is a fusion protein of tetracycline repressor (tetR) and the transcriptional activation domain derived from HSV VP16. The resulting tTA protein can bind to TRE sequences in the absence of tetracycline / DOX and activate gene expression. In the presence of tetracycline or DOX, they bind to tTA in a competitive way and render tTA incapable of binding to TRE, thereby preventing gene expression.

3)Tetracycline On System

Tetracycline On (Tet-On) System is developed based-on gene mutagenesis and creation of a reverse Tet repressor (rTetR). Compared with Tet-Off System, Tet-On System works similarly, but in the opposite fashion. Tet-On System involves a reverse tetracycline-controlled transactivator (rtTA) which is a fusion protein of rTetR and VP16. Unlike tTA that is capable of binding TRE without tetracycline or DOX, in a Tet-On system, rtTA can bind to TRE only when it is bound by a tetracycline or DOX. In other words, Tet-Off system activates gene expression in the absence of tetracycline or DOX, whereas Tet-On system activates gene expression in the presence of tetracycline or DOX.

Since the original first generation of Tet-On and Tet-Off Systems, new generations of this technology, Tet-Advanced System and Tet-on 3G System, have been developed through a series of improvements including gene mutagenesis and codon optimization. These new systems render higher and more stable expression levels in mammalian cells, increase sensitivity to tetracycline or DOX even further, and can reduce basal expression.

RGBiotech offers a wide collection of Tet inducible plasmid vectors with non-viral and viral backbones, all-in-one and dual vector formats. We also provide services for custom construction of Tet inducible plasmid vectors to help you achieve tetracycline-regulated expression of your target genes.

References

[1] Das AT, Tenenbaum L, Berkhout B. Tet-On Systems For Doxycycline-inducible Gene Expression. Curr Gene Ther. 2016;16(3):156-67.

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