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miRNA Sponge Clone

miRNA sponge technology was first introduced by Sharp’s laboratory in 2007 as a means to generate RNA molecules containing multiple, tandem binding sites for a miRNA seed family of interest and able to block a whole family of related miRNAs. This method is a useful tool for study of miRNA loss-of-function.

miRNAs in a same family often share a common seed sequence (positions of 2–8 of the miRNA, the major specificity determinant for target recognition).

The sponge mRNA contains multiple target sites complementary to a miRNA of interest. When the sponge vector is transfected into cells and the sponge mRNA is expressed at high levels, it specifically inhibits the activity of a family of miRNAs sharing a common seed.

RGBiotech offers high quality service for construction of miRNA sponge vector clones to assist researchers worldwide on their miRNA studies.

miRNA sponge

Features & Benefits

  • Compared with conventional AMOs, miRNA sponge plasmid constructs are easier to be amplified, purified and stably transfected into cells.
  • Multiple options for miRNA sponge vectors: non-viral/viral plasmid backbone, promoter, reporter gene, antibiotic selection marker, tandem repeat number etc.
  • Product may be delivered as plasmid or E.coli stock.
  • Cost effective and fast turnaround time

    • Tabel 1. Comparison of miRNA sponge and antagomir
    Comparison Item miRNA Sponge miRNA Antagomir
    Composition mRNA containing a 3' UTR RNA oligos with chemical modifications
    Plasmid backbone Yes No
    Reporter GFP, luciferase or other reporter proteins None
    Binding sites Multiple Single
    Specificity miRNA seed family Single miRNA
    Transient expression Yes Yes
    Stable expression Yes No
    Chromosomal insertions Yes No

    References

    [1]. EbertMS, Neilson JR, Sharp PA: MicroRNA sponges: Competitive inhibitors of small RNAs in mammalian cells. Nat Methods 2007, 4:721–726.
    [2]. Ebert MS, Sharp PA: MicroRNA sponges: progress and possibilities. RNA 2010, 16: 2043–2050.

    >Contact Us

    For more information about our miRNA services, please contact us at admin@rgbiotech.com.

     

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