PROTOCOL FOR PLASMID RECOVERY FROM FILTER PAPER

1. Use a clean razor blade and cut the marked circle area that contains the dried plasmid DNA.
2. Use clean forceps to transfer the circle into a 1.5 mL microcentrifuge tube.
3. Add 50 μL of TE buffer, vortex and briefly centrifuge.
4. Make sure that the filter paper is submerged in the TE buffer, incubate for 30 minutes at room temperature.
5. Vortex again and centrifuge the tube for a few seconds.
6. Pipette 2~10 μL of supernatant (recovered DNA) for use in transfecting E. coli through electroporation or chemical method.
7. Put the remainder of the filter paper/TE mix at -20°C for long-term storage.
Note: The recovered DNA is not recommended to use directly in any application other than E.coli transfection.

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