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siRNA Design & Synthesis siRNA (small interfering RNA), also known as short interfering RNA or silencing RNA, is a kind of double-stranded RNA oligo with two overhanging nucleotides on each strand. Once enters the cell, siRNA is processed by Dicer into smaller fragments. In most cases the antisense strand is incorporated into RNA-induced Silencing Complex (RISC), followed by target mRNA binding and cleavage. siRNA is an important tool for gene loss-of-function studies. RGBiotech’s unmatched siRNA services encompass design and synthesis of standard siRNA oligos, advanced siRNA oligos, siRNA control oligos, custom siRNA oligos. All siRNA oligos we provide are purified by HPLC. Standard siRNA Oligos Standard siRNA oligos are traditional unmodified double-stranded RNA molecules. For each target gene, RGBiotech design and synthesize four individual siRNAs. Customer can transfect individual siRNA into cells, or use siRNA pool to enhance interference efficiency. Advanced siRNA Oligos Advanced siRNA oligos are double-stranded RNA molecules with chemical modification (Please choose from Table 1). Compared with standard siRNAs, advanced siRNAs have higher stability, minimized side effects, and can exert interference in a longer period of time. siRNA Control Oligos siRNA controls should be included in siRNA experiments for reliable results. RGBiotech offers both negative and positive siRNA controls for RNAi experiments. 1) siRNA negative controls (Table 2)- Universal siRNA negative control is a double-stranded RNA oligo that has no homology with mammalian genes. - Scrambled siRNA negative control is a double-stranded RNA oligo that has the same base composition as the gene-specific siRNA but with an altered sequence. 2) siRNA positive controls (Table 3) siRNA positive controls are published siRNAs that have effective interference ability to specific genes. Custom siRNA Oligos RGBiotech can also synthesize siRNA oligos (<40 bp) designed by customers. A variety of modifications (Please see Table 1) are also available to improve siRNA stability, cellular uptake, cell tracking etc. Tabel 1. Chemical modifications for siRNA at RGBiotech
Table 2. siRNA negative controls at RGBiotech
Table 3. siRNA positive controls at RGBiotech
Features & Benefits
Cover all human, mouse and rat gene targets Contact Us For service inquiry, please contact us at admin@rgbiotech.com. We will contact you within 24 hours. |
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