Custom Retrovirus Production

Introduction

Recombinant retroviruses that commonly used in molecular biology are derived from MoMLV (Moloney Murine Leukemia Virus) or MSCV (Murine Stem Cell Virus). Recombinant retroviruses are replication incompetent because they do not contain genes required for replication and packaging. The VSV-G envelope allows recombinant retroviruses to infect cells of any species. Retroviruses and lentiviruses can randomly integrate their genomes into host cell genome. Thus they are often used for stable cell line generation. Unlike lentiviruses which can infect both dividing and non-dividing cells, retroviruses can only infect dividing cells.

RGBiotech offers complete services for producing high titer and high purity MMLV / MSCV based , VSV-G pseudotyped recombinant retroviruses. Please contact us at admin@rgbiotech.com for your special requirement.

Features

Service Workflow
- Communication with customers to discuss the fit-to-project solution
- Complete retrovirus production outline:
   -> sequence design and synthesis
   -> sequence subcloning into a retroviral transfer plasmid
   -> plasmid maxi-prep
   -> produce recombinant retrovirus in 293T cells by co-transfection of the transfer plasmid and the packaging plasmids
   -> retrovirus concentration and purification via ultra-centrifugation
   -> qPCR titration
- Technical support for adenovirus using

Case Studies

Figure1. Cav-1 KO MEF cells were immortalized and infected by mCav-1 recombinant retroviruses, followed by puromycin selection. Western blot analysis shows ~90% Cav-1 protein recovery in generated stable cell line compared with wild type level.

Figure2. H376, H103 and SG3 cells were transduced with retroviruses containing GFP / RFP.

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