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| Home -> Products & Services -> Custom Plasmid Vector Construction Services -> Custom Construction Service of Sleeping Beauty (SB) Transposon Expression Plasmid Vector | |||||||||||||||||||||||
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Custom Construction Service of Sleeping Beauty (SB) Transposon Expression Plasmid Vector The Sleeping Beauty (SB) transposon is an artificially reconstructed DNA transposon system, originally revived and optimized from fish genomes. As a non-viral gene delivery tool, the SB transposon has gained significant attention in gene therapy, cell engineering, and basic research due to its efficient and safe gene integration capabilities. Compared to traditional viral vectors, the SB transposon eliminates the complexity of viral packaging while reducing immunogenicity and insertion mutagenesis risks, making it a groundbreaking technology in gene delivery. RGBiotech offers Custom Construction Service of Sleeping Beauty (SB) Transposon Expression Plasmid Vector. If you need efficient and stable Sleeping Beauty transposon expression vectors, reach out today to obtain a personalized Sleeping Beauty transposon vector construction scheme! We offer end-to-end solutions from design to delivery, accelerating the progress of your research and clinical translation efforts! Why Choose RGBiotech? 1. Validated SB transposon backbone vectors supports the flexible configuration of universal / tissue-specific promoters, reporter genes, antibiotic resistance genes, multiple tags (Flag/Myc) etc.2. Vector Sequencing: Ensures accuracy of transposon sequences and inserted genes. 3. Provide personalized design 4. Delivery Content: Include the vector map, sequencing report, COA. 5. Professional Service Team 6. Advanced Equipment and Technology 7. More than 10 years of experience in vector construction 8. Fast Turnaround 9. Confidentiality: Full NDA compliance to protect intellectual property. Read More The Sleeping Beauty (SB) transposon system is a highly efficient non-viral gene delivery tool, originating from the Tc1/mariner superfamily of transposons found in the genome of salmon. Through artificial modification, scientists have fused and optimized the extinct transposase sequences, successively developing iterative versions such as SB10, SB100X, and the highly soluble hsSB. Among them, the gene integration efficiency of the SB100X transposase has been increased by 100 times compared to the initial generation, which is comparable to that of viral vectors. The introduction of hsSB has further optimized the protein stability, supporting electroporation delivery and freeze-thaw cycles, and significantly expanding its application range in difficult-to-transfect cells such as primary cells and stem cells. 1. Components of Sleeping Beauty Transposon 1) Transposon Vector: It contains the target gene expression cassette, antibiotic resistance genes / fluorescent reporter genes, and inverted repeat sequences (IR/DR), serving as the donor fragment for DNA integration.2) Transposase: SB100X or hsSB protein, which mediates the "cut-and-paste" type of genomic integration by recognizing the terminal sequences of the transposon. 2. Mechanism of Action The SB transposon operates via a "cut-and-paste" mechanism:1) Transposase Expression: Co-delivery of transposase (via mRNA or helper plasmid) and donor plasmid into target cells. 2) Transposon Excision: The transposase recognizes IR/DR sequences and excises the target gene from the donor plasmid. 3) Genomic Integration: The excised transposon randomly integrates into TA dinucleotide sites in the host genome through non-homologous end joining (NHEJ), enabling long-term stable expression. 3. Applications of Sleeping Beauty (SB) Transposon 1) Gene Function Research: Combined with the CRISPR-Cas9 technology, it can achieve gene overexpression, knockout, and conditional knockout, which is suitable for the mechanism research of tumors, metabolic diseases, etc.2) Cell Engineering: It is used for the long-term and stable modification of stem cells, T cells, etc., and supports the directional differentiation of iPSCs and the continuous expression of chimeric antigen receptors (CARs). 3) Transgenic Animal Models: It supports the rapid construction of large animal models (such as cows and pigs) with multiple gene modifications, and the efficiency is higher than that of the traditional pronuclear injection method. 4) Gene Therapy: It has been successfully applied to the preparation of CAR-T cells. For example, the CAR-T therapy targeting CD19 has a response rate of up to 90% in hematological tumors, and the cost is significantly lower than that of viral vectors. 4. Sleeping Beauty (SB) Transposon vs Viral Vectors
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