Custom Plasmid Vector Construction Services

Plasmid vectors are critical tools in molecular biology and biotechnology, and have been widely used for a variety of applications including cloning, expression, knockdown, knockout, mutation, replacement, knockin of target gene/miRNA/miRNA sponge/shRNA/gRNA/lncRNA/circularRNA etc.

RGBiotech possess a large collection of plasmid vectors and also offers a comprehensive platform for custom plasmid vector construction services. If you are interested in our services, please contact us at admin@rgbiotech.com for more information.

Why Choose RGBiotech for Custom Plasmid Vector Construction?

1. Professional Team

We have a professional team composed of senior molecular biology experts. They have rich experience in the field of plasmid vector construction and can provide personalized solutions according to different customer needs to ensure the efficiency and accuracy of the construction process.

2. Advanced Technology Platform

3. Strict Quality Control

4. Fast Delivery

5. High - Quality After - sales Service

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1. Plasmid Vector Introduction

2. Plasmid Vector Applications

1) Research Applications

In molecular biology research, plasmids are indispensable tools. They are widely used in gene cloning. By inserting the target gene into a plasmid vector and then introducing it into host cells, the large - scale amplification and expression of the target gene can be achieved, helping researchers to deeply study the function, structure, and regulatory mechanisms of genes. In gene - editing technologies such as CRISPR - Cas9, plasmids are used to carry the Cas9 protein gene and guide RNA sequences, precisely editing the target gene, which provides possibilities for tackling various genetic diseases and difficult - to - treat conditions.

2) Therapeutic Applications

In the field of gene therapy, plasmids can serve as vectors for gene delivery. By loading therapeutic genes into plasmid vectors and introducing them into specific cells of patients, abnormal genes can be repaired or replaced, thereby achieving the goal of treating diseases. Currently, plasmid vectors play a key role in gene - therapy clinical trials for some monogenic genetic diseases and tumors.

3. Classification of Plasmid Vectors

1) Cloning Plasmid Vectors

Mainly used for the cloning and amplification of target genes. They have multiple cloning sites, which facilitate the insertion of exogenous genes. At the same time, they have a high copy number, enabling them to replicate in large quantities in host cells and increase the yield of the target gene.

2) Expression Plasmid Vectors

Focus on the expression of target genes. In addition to basic replication elements, they also contain expression - regulatory elements such as promoters and terminators, which can initiate the transcription and translation processes of target genes in host cells and synthesize the corresponding protein products.

3) Shuttle Plasmid Vectors

These plasmids have replication origins and selection markers for two or more different host cells and can shuttle between different species of cells, such as Escherichia coli and yeast cells, Escherichia coli and mammalian cells, facilitating gene manipulation and expression research.

4. Plasmid Vector Elements

1) Replication Origin

It is a key element for the plasmid to replicate autonomously in host cells, determining the copy number and host range of the plasmid. Different replication origins have different replication characteristics. For example, high - copy - number replication origins can enable the plasmid to proliferate in large numbers in cells.

2) Selection Marker Genes

Common ones are antibiotic - resistance genes, such as ampicillin - resistance genes and kanamycin - resistance genes. By adding the corresponding antibiotics to the culture medium, only host cells that have successfully imported plasmids containing resistance genes can survive, which is convenient for screening and identifying positive clones.

3) Multiple Cloning Sites

It is a DNA sequence containing multiple restriction - endonuclease recognition sites, providing a convenient interface for the insertion of exogenous genes. Researchers can select appropriate restriction - enzyme sites for gene cloning according to experimental requirements.

4) Promoters and Terminators

The promoter is located upstream of the target gene and is the region where RNA polymerase binds to initiate the transcription process of the gene. The terminator is located downstream of the gene and indicates the termination of transcription, ensuring the integrity of mRNA transcription.

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