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| Home -> Products & Services -> Custom Plasmid Vector Construction Services -> Custom Construction Service of Tol2 Transposon Expression Plasmid Vector | ||
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Custom Construction Service of Tol2 Transposon Expression Plasmid Vector The Tol2 transposon originates from the Japanese medaka fish (Oryzias latipes) and is an autonomous DNA transposon. Due to its high transposition efficiency and broad host range, the Tol2 transposon has become an essential tool for gene function studies and the construction of transgenic animal models. RGBbiotech offers custom services for constructing Tol2 transposon plasmid vectors expressing your target gene / shRNA / miRNA / non-coding RNA to meet your scientific research and application needs. Through our professional services, you can obtain high-quality Tol2 transposon expression plasmid vectors to support your research. Contact us at admin@rgbiotech.com for more details! Why Choose RGBiotech? 1. Professional Team: Team members have a solid theoretical knowledge, a deep understanding of the Tol2 transposon system and vector construction technology, and can provide you with professional technical support and solutions.2. Extensive Experience: We have successfully completed multiple projects, and are familiar with various vector construction processes and optimization strategies. 3. Customized Service: According to your experimental purposes and specific requirements, different promoters, resistance genes, and reporter genes can be customized to create personalized expression vectors for you. 4. Strict Quality Inspection: The constructed vectors are strictly sequenced and verified to ensure the accuracy of the sequence, eliminating problems such as base mutations and incorrect ligation, and ensuring the smooth progress of the experiment. 5. Detailed Report: A detailed construction report will be provided, including sequencing data and vector maps. Read More The Tol2 transposon was initially discovered in medaka fish. It belongs to the hAT transposon superfamily and is the only known natural transposon system that can effectively transpose in vertebrates. It has shown great application potential in fields such as gene function research and gene therapy. 1.Features of Tol2 Transposon 1) High Transposition Efficiency: Tol2 exhibits high transposition activity in various organisms.2) Broad Host Range: Suitable for multiple species, including zebrafish, mice, rats, and more. 3) Large Capacity: Capable of carrying large exogenous gene fragments. 2. Composition of Tol2 Transposon The Tol2 transposon consists of the following main parts.1) Transposase Gene: Encodes the transposase. This enzyme recognizes the inverted terminal repeat sequences (ITR) at both ends of the transposon and catalyzes the excision and integration of the transposon. 2) Inverted Terminal Repeat Sequences (ITR): Located at both ends of the transposon, they are the action sites of the transposase and are crucial for the accurate transposition of the transposon. 3) Internal Coding Sequence: It can carry foreign genes, which are integrated into the host genome along with the transposition of the transposon and expressed. 3. Transposition Principle of Tol2 Transposon The transposition process of the Tol2 transposon involves three steps: transposase recognition, cleavage & insertion, repair and integration. Briefly, the transposase recognizes and binds to the ITR sequences at both ends of the Tol2 transposon, excises the transposon from its original position, and forms a transposase-transposon complex. Subsequently, this complex enters the nucleus through the nuclear localization signal and randomly integrates into the target site of the host genome to complete the transposition process. 4. Applications of Tol2 Transposon 1) Gene Function Research: By constructing reporter genes or interfering RNAs and other elements into the Tol2 transposon vector and transferring them into cell or animal models, the function and regulatory mechanism of genes can be studied.2) Preparation of Transgenic Animals: Utilizing the characteristic of the efficient integration of foreign genes by the Tol2 transposon, transgenic animal models such as mice and zebrafish can be prepared, which are used in research fields such as developmental biology and disease model construction. 3) Gene Therapy: As a potential gene delivery tool, therapeutic genes can be introduced into patient cells, providing a new approach for the treatment of monogenic genetic diseases and other diseases. 5. Tol2 Transposon vs Other Transposons 1) Wide Host Range: Compared with some transposons that can only function in specific species or cell types, the Tol2 transposon can transpose in a variety of vertebrates.2) Efficient Integration: Its transposition efficiency in vertebrate cells and individuals is relatively high, which is better than some transposon systems and can achieve gene integration more effectively. 3) In some cell types, there may be a certain insertion preference. Although there is no strict sequence specificity, the integration site is not completely random. 6. Tol2 Transposon vs Viral Vectors 1) High Safety: It does not have the pathogenicity and immunogenicity of viruses, reducing the possibility of triggering immune responses and other safety risks during application.2) Simple Operation: The construction and use process is relatively simple, and there is no need for a complex virus packaging process, saving time and cost. 3) Larger Vector Capacity: It can accommodate the insertion of larger fragments of foreign genes, meeting the needs of more gene manipulation. 4) In some cases, the transfection efficiency is lower than that of viral vectors. Especially for some difficult-to-transfect cell types, it may be necessary to optimize the transfection conditions to improve the efficiency. |
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