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| Home -> Products & Services -> Cell Immortalization Plasmid Vectors -> [HPV16 E6-E7] Expression Plasmid Vector | ||
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[HPV16 E6-E7] Expression Plasmid Vector Item No.: PIM-003Shipping: 4 μg Storage: -20°C The genome of human papillomavirus type 16 (HPV16) contains seven early genes, of which E6 and E7 have been associated with HPV-induced immortalization. Several studies have shown that the E6 and E7 genes of HPV16 can cooperate to immortalize a broad spectrum of cell types including keratinocytes, fibroblasts etc. The E6 and E7 proteins are know to bind to tumor suppressor gene products p53 and retinoblastoma (RB), respectively. The interactions result in a mutant p53 phenotype and impedes the interaction of Rb with several proteins to disrupt the cell cycle. RGBiotech provides various types of plasmid vectors expressing HPV16-E6/E7 to assist your scientific research work in the field of primary cell immortalization. Please contact us for more product information as well as the price. Highights -Our large collection of empty vector backbones can always allow you to find the fit-for-project plasmid vectors.-Sequencing validation to make sure zero mutation, -Competitive price APPlications Production of viral particles by packaging viral-based plasmid vectorsGeneration of immortalized cell line References [1] Hawley-Nelson P, Vousden KH, Hubbert NL, Lowy DR, Schiller JT. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO J. 1989 Dec 1;8(12):3905-10.[2] Sedman SA, Barbosa MS, Vass WC, Hubbert NL, Haas JA, Lowy DR, Schiller JT. The full-length E6 protein of human papillomavirus type 16 has transforming and trans-activating activities and cooperates with E7 to immortalize keratinocytes in culture. J Virol. 1991 Sep;65(9):4860-6. [3] Shiga T, Shirasawa H, Shimizu K, Dezawa M, Masuda Y, Simizu B. Normal human fibroblasts immortalized by introduction of human papillomavirus type 16 (HPV-16) E6-E7 genes. Microbiol Immunol. 1997;41(4):313-9. [4] Shiga T, Shirasawa H, Shimizu K, Dezawa M, Masuda Y, Simizu B. Normal human fibroblasts immortalized by introduction of human papillomavirus type 16 (HPV-16) E6-E7 genes. Microbiol Immunol. 1997;41(4):313-9. |
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