Kinase Expression Plasmid Vectors

Protein kinases are groups of enzymes that are involved in protein phosphorylation, including tyrosine kinases, serine/threonine kinases, MAP2K and GSK families. Protein kinases play key roles in the signaling cascade and regulate diverse biological processes such as growth, differentiation, metabolism, apoptosis, proliferation, cell cycle and motility etc. The entire set of protein kinases encoded in the genome of an organism is termed as Kinome. The human kinome contains over five hundreds of protein kinase genes. Many of these kinases are associated with human cancer initiation and progression through ways like mis-regulated expression and/or amplification/overexpression, aberrant phosphorylation, mutation(s), chromosomal translocation, and epigenetic regulation. The development of small-molecule kinase inhibitors that can block the constitutive oncogenic activation in cancer cells has become a promising approach in cancer therapies. Protein kinases have become the second most targeted group of drug targets, after the G-protein-coupled receptors (GPCRs). So far, several small-molecule kinase inhibitors have received FDA approval for the treatment of many malignancies such as breast cancer and lung cancer, and many more are in late stages of clinical development and are likely to be approved in the coming years.

RGBiotech offers a large list of kinase expression plasmid vectors which cover almost all hot kinase targets such as EGFR (WT and mutants), ERBB2/HER2 (WT and mutants), BCR-ABL fusion mutants, EML4-ALK fusion mutants, KRAS mutants, ETV6 fusion mutants, FLT3 mutants, MET mutants, FGFR mutants and so on. Our kinase expression plasmid vectors offer different options on the vector backbone: non-viral, lentiviral, or retroviral backbone. The kinase expression plasmid vectors can be used for mammalian cell electroporation, lipo-transfection, downstream recombinant virus production and mammalian cell transduction to establish specific kinase expression stable cell lines. The established kinase stable cell lines can be used for in vitro screening of kinase-targeted drugs.

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