pComb3XSS

INTRODUCTION

pComb3XSS is a phagemid vector which is designed for phage display of full Fab, scFv, peptide and other proteins. pComb3XSS contains two stuffers in light chain and heavy chain cloning regions, a 1200bp stuffer bounded by SacI and XbaI restriction sites and a 300bp stuffer bound by XhoI and SpeI restriction sites. The two stuffers can be removed by SfiI digest so that non-Fab genes of interest can be cloned. 6xHis and HA tags allow for purification and detection. The gene for phage protein pIII can be removed by SpeI/NheI digest for soluble expression. Alternatively, an amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning.

VECTOR INFORMATION

Figure 1. pComb3XSS Vector Map

RESEARCH REFERENCES

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