 |
|
|
| Home -> Products & Services -> Phage Display Plasmids -> pComb3XTT | ||
| Quick Inquiry |
pComb3XTT Item No.: PPD005Shipping: 2 μg Storage: -20°C INTRODUCTION pComb3XTT is a phagemid vector that contains the human Fab to tetanus toxin. The human Fab to tetanus toxin which can be used as a template to amplify the kappa light chain constant region and Fd for construction of chimeric antibody libraries. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. VECTOR INFORMATION pComb3XTT plasmid sequence
Figure 1. pComb3XTT Vector Map RESEARCH REFERENCES Methods for the generation of chicken monoclonal antibody fragments by phage display. Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. doi: 10.1016/s0022-1759(00)00221-0. PMID: 10986398. |
|
|
Products & Services Resources | ||
|
Home Products & Services Support About Us Contact Us |
Promotions Flyers Brochures Publications News & Events |
Terms & Conditions Privacy Disclaimers |
Contact Us EMAIL: admin@rgbiotech.com |
||
| © RGBiotech All Rights Reserved. | |||||