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pComb8 Item No.: PPD002Shipping: 2 μg Storage: -20°C INTRODUCTION As a phagemid, pComb8 is similar to pComb3 except that the geneIII sequence is replaced with geneVIII sequence. Production of phages containing pComb8 needs the presence of helper phages, such as VCSM13. Like pComb3, for Fab display, the heavy chain sequence is cloned into the XhoI/SpeI restriction sites and the light chain is cloned into the SacI/XbaI restriction sites respectively. ProteinVIII is naturally multi-copied so pComb8 displaying is multi-valent compared with the pCOMB3 system. pComb8 can also be used for displaying other proteins of interest. VECTOR INFORMATION pComb8 plasmid sequence
Figure 1. pComb8 Vector Map RESEARCH REFERENCES Assembly of combinatorial antibody libraries on phage surfaces: The gene III site. Barbas III, C.F., Kang, A.S., Lerner, R.A. and Benkovic, S.J. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82. PMID: 1896445Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Huse WD, Sastry L, Iverson SA, Kang AS, Alting-Mees M, Burton DR, Benkovic SJ, Lerner RA. Science. 1989 Dec 8;246(4935):1275-81. PMID: 2531466 λ ZAP: a bacteriophage X expression vector with in vivo excision properties. Jay M.S., Joseph M.F., Joseph A.S. and William D.H. Nucleic Acids Res. 1988 Aug 11; 16(15): 7583–7600. PMCID: PMC338428 |
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