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pJF3H Plasmid Vector Item No.: PPD009Shipping: 4 μg Storage: -20°C INTRODUCTION pJF3H is a phagemid vector which is designed for phage display of cDNA libraries. pJF3H contains ompA and pelB leaders, Jun, Fos and a cloning site for cDNA expression. The cDNA library is cloned into the C-terminal of Fos and expressed as a fusion protein. The pIII gene product is at the C-terminal of Jun and expressed as a fusion protein. The ompA and pelB lead the fusion proteins to the periplasm where they assembly through Jun/Fos interaction. RGBiotech offers pJF3H plasmid vector validated by full length sequencing to make sure sequence accuracy. Please contact us at admin@rgbiotech.com for more information. VECTOR INFORMATION pJF3H plasmid sequence
Figure 1. pJF3H Vector Map RESEARCH REFERENCES Phage Display: A Laboratory Manual. Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001)Phage display to identify Nedd8-mimicking peptides as inhibitors of the Nedd8 transfer cascade. Zhao B, Zhang K, Villhauer EB, Bhuripanyo K, Kiyokawa H, Schindelin H, Yin J. Chembiochem. 2013 Jul 22;14(11):1323-30. doi: 10.1002/cbic.201300234. Epub 2013 Jul 3. PMID: 23824602; PMCID: PMC3822002. Profiling the cross reactivity of ubiquitin with the Nedd8 activating enzyme by phage display.Zhao B, Zhang K, Bhuripanyo K, Choi CH, Villhauer EB, Li H, Zheng N, Kiyokawa H, Schindelin H, Yin J. PLoS One. 2013 Aug 1;8(8):e70312. doi: 10.1371/journal.pone.0070312. PMID: 23936405; PMCID: PMC3731359. Development and Application of Yeast and Phage Display of Diverse Lanthipeptides. Hetrick KJ, Walker MC, van der Donk WA. ACS Cent Sci. 2018 Apr 25;4(4):458-467. doi: 10.1021/acscentsci.7b00581. Epub 2018 Mar 28. PMID: 29721528; PMCID: PMC5920614. |
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